CyanoLyase

CpcS family

Description

Two members of the CpcS family were biochemically characterized: Nostoc sp. PCC7120 CpcS-III and Synechococcus sp. PCC 7002 CpcS-I. Both bind a PCB at Cys-82 (consensus numbering) of alpha and beta-allophycocyanin (ApcA, B, D, F), beta-phycocyanin (CpcB/RpcB and beta-phycoerythrocyanin (PecB) in vivo (Zhao et al., 2007). In vitro, CpcS can also catalyze the attachment of PEB to Cys-84 of alpha and beta-PEI (CpeA-B) and is thus is a nearly universal lyase with respect to apoproteins but is highly specific of this binding site.

References

  • Saunée et al., 2008. Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to Cys-82 of beta-phycocyanin and Cys-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002 J Biol Chem, 283, 7513-7522.
  • Shen et al., 2008. Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phycocyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits J Biol Chem, 283, 7503-7512.
  • Zhao et al., 2007a. Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins Proc Natl Acad Sci USA, 104, 14300-14305.
  • Zhao et al., 2007b. Lyase activities of CpcS- and CpcT-like proteins from Nostoc PCC7120 and sequential reconstitution of binding sites of phycoerythrocyanin and phycocyanin beta-subunits J Biol Chem, 282, 34093-34103.

Gene Locus tag Genbank Species Strain PBP in rods Pigment type Remark Length Last change
CpcS family AM1_4215 YP_001518512.1 Acaryochloris marina MBIC11017 (AM1) PC 1 1st copy, strange position in phylogeny. By blast: closer from CpcSI. 197 08-20-2012
CpcS family AM1_C0217 YP_001521653.1 Acaryochloris marina MBIC11017 (AM1) PC 1 2nd copy,strange position in phylogeny. By blast: closer from CpcSI. Located on preb3 plasmid. 208 08-20-2012
CpcS family CMR092C Cyanidioschyzon merolae 10D PC 1 long N-term compared to cyanobacterial CpcS sequences, found by tblastn 405 02-21-2012
CpcS-I subfamily S7335_4321 ZP_05037881.1 Synechococcus sp. PCC7335 PC+PEI CA3 This sequence is closely related to CpcS-I, but the genome of PCC7335 apparently does not contain any CpcU-I, possibly because it is incomplete. 200 10-03-2012
CpcS-I subfamily SYNPCC7002_A1822 YP_001735066.1 Synechococcus sp. PCC7002 PC 1 198 09-26-2011
CpcS-I subfamily slr2049 NP_440437.1 Synechocystis sp. PCC6803 (ATCC27184) PC 1 192 09-26-2011
CpcS-I subfamily AmaxDRAFT_2794 ZP_03273970.1 Arthrospira (Spirulina) maxima CS-328 PC 1 197 09-26-2011
CpcS-I subfamily CwatDRAFT_6342 ZP_00514111.1 Crocosphaera watsonii WH8501 PC+PEI 197 09-26-2011
CpcS-I subfamily cce_3207 YP_001804621.1 Cyanothece sp. ATCC51142 PC 1 198 09-26-2011
CpcS-I subfamily PCC7424_3509 YP_002378769.1 Cyanothece sp. PCC7424 PC+PEI 2 197 09-26-2011
CpcS-I subfamily PCC8801_1352 YP_002371570.1 Cyanothece sp. PCC8801 PC+PEI 2 200 09-26-2011
CpcS-I subfamily Cyan8802_1382 YP_003137135.1 Cyanothece sp. PCC8802 PC 1 200 09-26-2011
CpcS-I subfamily CY0110_04081 ZP_01730273.1 Cyanothece sp. CCY0110 PC 1 197 09-26-2011
CpcS-I subfamily Cyan7822_2533 YP_003887780.1 Cyanothece sp. PCC7822 PC+PEI 2 198 09-26-2011
CpcS-I subfamily gvip161 NP_924137.1 Gloeobacter violaceus PCC7421 PC+PEI 2 strange position in phylo 201 11-24-2011
CpcS-I subfamily GL6909_5285 Gloeothece sp. PCC6909/1 PC+PEI 2 198 05-31-2011
CpcS-I subfamily L8106_24665 ZP_01619909.1 Lyngbya aestuarii CCY9616 (PCC8106/CCY8106) PC 1 No other start codon before this sequence 197 09-26-2011
CpcS-I subfamily MAE_48330 YP_001659847.1 Microcystis aeruginosa NIES-843 PC 1 A stop codon, then 4aa, then the start codon 193 09-26-2011
CpcS-I subfamily Npun_F3809 YP_001867136.1 Nostoc punctiforme ATCC29133 (PCC73102) PC+PEI 2 CpcSIII in Schluchter et al., 2010? 206 09-26-2011
CpcS-I subfamily Synpcc7942_2031 YP_401048.1 Synechococcus elongatus PCC7942 PC 1 193 09-26-2011
CpcS-I subfamily syc2064_d YP_172774.1 Synechococcus elongatus PCC6301 (ATCC 27144) PC 1 193 11-25-2011
CpcS-I subfamily Tery_3908 YP_723414.1 Trichodesmium erythraeum IMS101 PC+PEI N-term shortened (5 aa removed: MEYRL) 196 08-30-2012
CpcS-I subfamily OSCI_700007 ZP_07109069.1 Oscillatoria sp. PCC6506 (PCC9029/ATCC29081 /UTEX 1547) PC 1 197 10-19-2011
CpcS-I subfamily LYNGBM3L_55940 ZP_08427741.1 Lyngbya majuscula 3L PC+PEI 2 216 10-19-2011
CpcS-I subfamily MicvaDRAFT_5174 ZP_08495221.1 Microcoleus vaginatus FGP-2 PC 1 198 10-20-2011
CpcS-I subfamily IPF_855 CAO87966.1 Microcystis aeruginosa PCC7806 PC 1 193 10-20-2011
CpcS-I subfamily NIES39_A02700 BAI88109.1 Arthrospira (Spirulina) platensis NIES-39 PC 1 197 10-20-2011
CpcS-I subfamily Cy51472DRAFT_4686 ZP_08975889.1 Cyanothece sp. ATCC51472 PC 1 198 01-10-2012
CpcS-I subfamily AplaP_010100006702 ZP_06381354.1 Arthrospira (Spirulina) platensis Paraca PC 1 197 01-24-2012
CpcS-I subfamily ACCM5_010100032969 ZP_09252138.1 Acaryochloris sp. CCMEE5410 APC only 196 08-24-2012
CpcS-I subfamily CWATWH0003_0565 EHJ14767.1 Crocosphaera watsonii WH0003 PC+PEI 197 02-22-2012
CpcS-I subfamily MICAI_250011 ZP_10228158.1 Microcystis sp. T1-4 PC+PEI 2 193 09-16-2012
CpcS-I subfamily MICAG_860004 CCI29527.1 Microcystis aeruginosa PCC9808 PC 1 193 08-22-2012
CpcS-I subfamily MICAB_3830008 CCH97799.1 Microcystis aeruginosa PCC9717 PC 1 193 08-23-2012
CpcS-I subfamily MICAK_3480004 CCI37760.1 Microcystis aeruginosa PCC9701 PC 1 193 08-23-2012
CpcS-I subfamily MICAC_4950001 CCI03754.1 Microcystis aeruginosa PCC9443 PC 1 193 08-23-2012
CpcS-I subfamily MICCA_980010 ZP_18815604.1 Microcystis aeruginosa PCC9432 PC 1 193 04-22-2013
CpcS-I subfamily MICAD_310004 CCI08315.1 Microcystis aeruginosa PCC7941 PC 1 193 08-23-2012
CpcS-I subfamily Osc7112_3154 YP_007115956.1 Oscillatoria nigro-viridis PCC7112 PC+PEI 2 198 04-22-2013
CpcS-I subfamily Sta7437_2864 YP_007133350.1 Stanieria cyanosphaera PCC7437 PC+PEI 199 04-22-2013
CpcS-I subfamily O53_3911 ZP_20934710.1 Microcystis aeruginosa TAIHU98 PC 1 193 04-22-2013
CpcS-I subfamily Lepto7376_3399 YP_007072440.1 Leptolyngbya sp. PCC7376 PC+PEI 200 04-23-2013
CpcS-I subfamily Cyast_1790 YP_007165395.1 Cyanobacterium stanieri PCC7202 PC 196 04-23-2013
CpcS-I subfamily Ple7327_2633 YP_007081472.1 Pleurocapsa sp. PCC7327 PC+PEI 198 04-23-2013
CpcS-I subfamily Xen7305DRAFT_00011740 ZP_21056994.1 Xenococcus sp. PCC7305 PC+PEI 199 04-23-2013
CpcS-I subfamily PCC7418_3218 YP_007169548.1 Halothece sp. PCC7418 PC 193 04-23-2013
CpcS-I subfamily GLO73106DRAFT_00025640 ZP_21050308.1 Gloeocapsa sp. PCC73106 PC+PEI 197 04-23-2013
CpcS-I subfamily Dacsa_0540 YP_007170680.1 Dactylococcopsis salina PCC8305 194 04-23-2013
CpcS-I subfamily Pse7367_3816 YP_007083475.1 Pseudanabaena sp. PCC7367 PC+PEI 208 04-23-2013
CpcS-I subfamily Mic7113_2585 YP_007121784.1 Microcoleus sp. PCC7113 PC+PEI 2 205 04-23-2013
CpcS-I subfamily ZP_18908102 ZP_18908102.1 Leptolyngbya sp. PCC7375 PC+PEI 2 204 04-23-2013
CpcS-I subfamily Pse7429DRAFT_3448 ZP_21067785.1 Pseudanabaena sp. (biceps) PCC7429 PC 200 04-24-2013
CpcS-I subfamily Oscil6304_1130 YP_007084776.1 Oscillatoria acuminata PCC6304 (SAG 1449-3) PC 203 04-29-2013
CpcS-I subfamily Cyan10605_0823 YP_007160999.1 Cyanobacterium aponimum PCC10605 194 04-29-2013
CpcS-I subfamily Cha6605_1319 YP_007096040.1 Chamaesiphon minutus PCC6605 PC+PEI 200 04-29-2013
CpcS-I subfamily LepboDRAFT_3190 Leptolyngbya boryana PCC6306 PC 195 06-11-2013
CpcS-I subfamily Osc10802DRAFT_6754 Oscillatoria sp. PCC10802 PC+PEI 204 06-12-2013
CpcS-I subfamily Cal6303_1002 YP_007136039.1 Calothrix sp. PCC6303 PC+PEI 2 196 06-20-2013
CpcS-I subfamily Lep6406DRAFT_00042270 ZP_21044550.1 Leptolyngbya sp. PCC6406 PC+PEC 198 06-20-2013
CpcS-II subfamily sync_2487 YP_731681.1 Synechococcus sp. CC9311 PC+PEI+PEII 3d/CA4 203 09-26-2011
CpcS-II subfamily Syncc9605_2286 YP_382575.1 Synechococcus sp. CC9605 PC+PEI+PEII 3c 197 09-26-2011
CpcS-II subfamily SH8109_0894 ZP_05790285.1 Synechococcus sp. WH8109 PC+PEI+PEII 3b 197 09-26-2011
CpcS-II subfamily SYNW0315 NP_896410.1 Synechococcus sp. WH8102 PC+PEI+PEII 3c 198 09-26-2011
CpcS-II subfamily Syncc9902_0400 YP_376414.1 Synechococcus sp. CC9902 PC+PEI+PEII 3d/CA4 198 09-26-2011
CpcS-II subfamily BL107_17200 ZP_01468673.1 Synechococcus sp. BL107 PC+PEI+PEII 3d/CA4 198 09-26-2011
CpcS-II subfamily SynWH7803_2155 YP_001225878.1 Synechococcus sp. WH7803 PC+PEI+PEII 3a 196 09-26-2011
CpcS-II subfamily WH7805_11643 ZP_01122710.1 Synechococcus sp. WH7805 PC+PEI 2 196 09-26-2011
CpcS-II subfamily RS9917_07715 ZP_01081133.1 Synechococcus sp. RS9917 PC 1 206 09-26-2011
CpcS-II subfamily RS9916_35312 ZP_01471093.1 Synechococcus sp. RS9916 PC+PEI+PEII 3d/CA4 202 09-26-2011
CpcS-II subfamily WH5701_15871 ZP_01084219.1 Synechococcus sp. WH5701 PC 1 Frameshifted; join(2811210..2811479,2811479..2811838) 209 09-26-2011
CpcS-II subfamily SynRCC307_2024 YP_001228280.1 Synechococcus sp. RCC307 PC+PEI+PEII 3b 196 09-26-2011
CpcS-II subfamily SCB01_010100002463 ZP_07972490.1 Synechococcus sp. CB0101 PC 1 MAACDHRCTRRAA removed at the beginning 203 11-04-2011
CpcS-II subfamily SCB02_010100000529 ZP_07969387.1 Synechococcus sp. CB0205 PC+PEI 2 201 11-04-2011
CpcS-II subfamily CPCC7001_2732 ZP_05046541.1 Cyanobium sp. PCC7001 PC 1 200 09-26-2011
CpcS-II subfamily Syn8016DRAFT_2357 ZP_08957012.1 Synechococcus sp. WH8016 PC+PEI+PEII 3a 203 11-04-2011
CpcS-II subfamily PCC_0659 YP_002049288.1 Paulinella chromatophora M0880 PC 1 Protoscan score is not very high, but it matches CpcS-II best 198 08-30-2012
CpcS-II subfamily SynWH8020_CpcSII Synechococcus sp. WH8020 PC+PEI+PEII 3d/CA4 From unpublished WH8020 genome 203 08-27-2012
CpcS-II subfamily Cyagr_1540 YP_007046040.1 Cyanobium gracile PCC6307 PC 206 04-29-2013
CpcS-III subfamily alr0617 NP_484661.1 Anabaena (Nostoc) sp. PCC7120 PC+PEC 188 09-26-2011
CpcS-III subfamily Ava_4549 YP_325042.1 Anabaena variabilis ATCC29413 (PCC7937) PC+PEC 188 09-27-2011
CpcS-III subfamily Aazo_1709 YP_003720981.1 Anabaena (Nostoc) azollae 0708 PC+PEC 191 09-26-2011
CpcS-III subfamily Cyan7425_2083 YP_002482807.1 Cyanothece sp. PCC7425 PC 1 188 09-26-2011
CpcS-III subfamily FJSC11DRAFT_1071 ZP_08984865.1 Fischerella sp. (Mastigocladus laminosus) JSC-11 PC+PEC 187 11-04-2011
CpcS-III subfamily MC7420_7485 ZP_05023633.1 Microcoleus chthonoplastes PCC7420 PC+PEC 186 09-26-2011
CpcS-III subfamily N9414_02356 ZP_01632464.1 Nodularia spumigena CCY9414 PC 1 188 09-26-2011
CpcS-III subfamily CYB_2841 YP_479027.1 Synechococcus sp. JA-2-3B'a(2-13) PC 1 190 09-26-2011
CpcS-III subfamily CYA_2807 YP_476172.1 Synechococcus sp. JA-3-3Ab PC 1 187 09-26-2011
CpcS-III subfamily tll1699 NP_682489.1 Thermosynechococcus elongatus BP-1 PC 1 3D structure available (pdb:3BDR). N-term shortened (4 aa removed) 178 08-29-2012
CpcS-III subfamily CRD_00076 ZP_06303581.1 Raphidiopsis brookii D9 PC 1 Contains a group II intron containing an orf (gi|282895443|ref|ZP_06303580.1|)
This sequence is the fusion of CRD_00076 and CRD_00074 (KHEGSTVLVTVPDLLKPGEGKLLRE added before CRD_00074)
197 11-02-2011
CpcS-III subfamily CRC_02172 ZP_06308247.1 Cylindrospermopsis raciborskii CS-505 PC 1 Fusion of CRC_02170 and CRC_02172. Contains a group II intron (same as D9 strain). KHEGSTVLVTVPDLLKPGEGKLLRE added before CRC_02172 197 12-02-2011
CpcS-III subfamily GEI7407_2263 YP_007109790.1 Geitlerinema sp. PCC7407 PC 186 06-20-2013
CpcS-III subfamily Cri9333_3248 YP_007143590.1 Crinalium epipsammum PCC9333 PC 186 06-20-2013
CpcS-III subfamily Glo7428_4424 YP_007130027.1 Gloeocapsa sp. PCC7428 PC 187 04-22-2013
CpcS-III subfamily Syn7509DRAFT_00025220 ZP_21040972.1 Synechocystis sp. PCC7509 PC 185 04-22-2013
CpcS-III subfamily Anacy_0109 YP_007154628.1 Anabaena cylindrica PCC7122 PC+PEC 190 04-23-2013
CpcS-III subfamily Cylst_0830 YP_007145836.1 Cylindrospermum stagnale PCC7417 PC+PEC 191 04-23-2013
CpcS-III subfamily Nos7524_3943 YP_007077313.1 Nostoc sp. PCC7524 PC+PEC 187 04-23-2013
CpcS-III subfamily Riv7116_2426 YP_007055488.1 Rivularia sp. PCC7116 PC+PEC 188 04-23-2013
CpcS-III subfamily Chro_1676 YP_007091064.1 Chroococcidiopsis thermalis PCC7203 PC+PEC 186 04-23-2013
CpcS-III subfamily Nos7107_3741 YP_007051452.1 Nostoc sp. PCC7107 PC+PEC 187 04-29-2013
CpcS-III subfamily Cal7507_3605 YP_007066831.1 Calothrix sp. PCC7507 PC+PEC 188 04-29-2013
CpcS-III subfamily Fis9431DRAFT_3727 Fischerella sp. PCC9431 PC+PEC 187 06-12-2013
CpcS-III subfamily FIS9605DRAFT_06979 Fischerella sp. PCC9605 PC+PEC 188 06-12-2013
CpcS-III subfamily FisPCC73103_3408 Fischerella muscicola PCC73103 (SAG 1427-1) PC+PEC 187 06-12-2013
CpcS-III subfamily Scytonema_PCC7110_joined_11887 Scytonema hofmanni PCC7110 PC+PEC 189 06-12-2013
CpcS-III subfamily PCC9339DRAFT_01730 Fischerella sp. PCC9339 PC+PEC 187 06-12-2013
CpcS-III subfamily Ana7108_2980 Anabaena sp. PCC7108 PC+PEC 191 06-12-2013
CpcS-III subfamily Chloroglopsis_PCC9212_joined_7598 Chlorogloeopsis sp. PCC9212 PC+PEC 189 06-12-2013
CpcS-III subfamily UYC_05286 Chlorogloeopsis fritschii PCC6912 PC+PEC 189 06-12-2013
CpcS-III subfamily FisPCC7414_4393 Fischerella muscicola PCC7414 PC+PEC 187 06-12-2013
CpcS-III subfamily Fischerella_sp._PCC7521_3615 Fischerella thermalis PCC7521 PC+PEC 187 06-12-2013
CpcS-III subfamily Syn6312_0613 YP_007060377.1 Synechococcus sp. PCC6312 PC 186 06-12-2013
CpcS-III subfamily Syn7502_02665 YP_007106758.1 Synechococcus sp. PCC7502 PC 172 06-20-2013
CpcS-III subfamily Mic7113_0798 YP_007120112.1 Microcoleus sp. PCC7113 PC+PEI 2 Found both CpcS-I and CpcS-III in this strain? 188 06-20-2013

Model strain Model strains, Marine strains

Biogenouest Station biologique de Roscoff ANR

Copyright © 2005-2012 Biogenouest® - Legal notice - Contact : Contact GenOuest

Hosted by the GenOuest bioinformatics platform