CyanoLyase

Characterized or predicted function of phycobilin lyases

This table lists the characterized (roman) or predicted (italics) function of phycobilin lyases in vivo, in terms of their:

  • chromophore bound: phycocyanobilin (PCB) or phycoerythrobilin (PEB)
  • enzymatic activity: lyase or lyase-isomerase. Note that a PCB lyase-isomerase will bind a PCB and isomerize it into phycoviolibilin (PVB) and that a PEB lyase-isomerase will bind a PEB and isomerize it into phycourobilin (PUB).
  • Substrate: apoprotein targeted by the enzyme
  • Cysteinyl binding sites: positions where chromophores are bound. Numbers in the table correspond to consensus numbering (Scheer et al., 2008). Note that marine Synechococcus for instance generally have different positions than indicated (i.e. Cys-81 on all APC subunits; Cys-82 and 153 on CpcB/RpcB; Cys-82 and 139 on CpeA; Cys-50/61, 82 and 165 on CpeB).

It is worth noting that many phycobilin lyases have a broader enzymatic activity in vitro, i.e. in heterologous, multi-plasmid expression systems in Escherichia coli (see e.g. Blot et al., 2009).

Phycobiliprotein Allophycocyanin Phycocyanin Phycoerythrocyanin Phycoerythrin I Phycoerythrin II Phycoerythrin III
Apoprotein name ApcA ApcB ApcD ApcF CpcA/RpcA CpcB/RpcB CpcB/RpcB PecA PecB PecB CpeA CpeA CpeB CpeB CpeB - MpeA MpeA MpeA MpeB MpeB MpeB - PpeA PpeB PpeB PpeB -
Consensus binding site α-82 β-82 αB-82 β18-82 α-84 β-84 β-155 α-84 β-84 β-155 α-84 a-143 β-50/61 β-84 β-155 unknown α-75 α-83 α-140 β-50/61 β-82 β-159 unknown α-73 β-61 β-82 β-163 unknown
Function

PCB lyase

CpcS-I + CpcU-I

CpcS-II + CpcU-II ?

CpcS-III

CpcS-I + CpcU-I

CpcS-II + CpcU-II ?

CpcS-III

CpcS-I + CpcU-I

CpcS-II + CpcU-II ?

CpcS-III

CpcS-I + CpcU-I

CpcS-II + CpcU-II ?

CpcS-III

CpcE-I + CpcF-I

CpcE-II + CpcF-II ?

CpcS-I + CpcU-I

CpcS-II + CpcU-II ?

CpcS-III

CpcT

CpcS-III

CpcT

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PEB lyase

RpcE + RpcF ?

RpcT ?

CpeY + CpeZ

CpeF ?

CpeS-I

CpeS-II ?

CpeT ?

CpeU ?

CpeY + CpeZ ?

CpeF ?

CpeS-II ?

CpeT ?

MpeU ?

CpeY + CpeZ ?

CpeF ?

CpeS-II ?

CpeS-ProHL

CpeT ?

PCB lyase-isomerase

PecE + PecF

PEB lyase-isomerase

RpcG

Unknown PEB lyase-isomerase ?

Unknown PEB lyase-isomerase ?

MpeY ?

Unknown PEB lyase-isomerase ?

MpeZ

MpeW ?

Unknown PEB lyase-isomerase ?

MpeY ?

Unknown PEB lyase-isomerase ?

Unknown PEB lyase-isomerase ?

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