CpcS family
Description
Two members of the CpcS family were biochemically characterized: Nostoc sp. PCC7120 CpcS-III (Zhao et al. 2007b) and Synechococcus sp. PCC 7002 CpcS-I (Saunée et al. 2008, Shen et al. 2008). Both bind a PCB at Cys-82 (consensus numbering) of alpha and beta-allophycocyanin (ApcA, B, D, F), beta-phycocyanin (CpcB/RpcB and beta-phycoerythrocyanin (PecB) in vivo (Zhao et al., 2007a,b). In vitro, CpcS can also catalyze the attachment of PEB to Cys-84 of alpha and beta-PEI (CpeA-B) and is thus is a nearly universal lyase with respect to apoproteins but is highly specific of this binding site (Zhao et al. 2007a).
Motif
A motif characterising this family is available.
References
- Saunée et al., 2008. Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to Cys-82 of beta-phycocyanin and Cys-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002 J Biol Chem, 283, 7513-7522.
- Shen et al., 2008. Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phycocyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits J Biol Chem, 283, 7503-7512.
- Zhao et al., 2007a. Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins Proc Natl Acad Sci USA, 104, 14300-14305.
- Zhao et al., 2007b. Lyase activities of CpcS- and CpcT-like proteins from Nostoc PCC7120 and sequential reconstitution of binding sites of phycoerythrocyanin and phycocyanin beta-subunits J Biol Chem, 282, 34093-34103.
Showing only unsure genes
| Gene | Locus tag | Genbank | Species | Strain | PBP in rods | Pigment type | Remark | Length | Last change |
|---|---|---|---|---|---|---|---|---|---|
| CpcS family | AM1_4215 | YP_001518512.1 | Acaryochloris marina | MBIC11017 (AM1) | PC | 1 | 1st copy, strange position in phylogeny. By blast: closer from CpcSI. | 197 | 08-20-2012 |
| CpcS family | AM1_C0217 | YP_001521653.1 | Acaryochloris marina | MBIC11017 (AM1) | PC | 1 | 2nd copy,strange position in phylogeny. By blast: closer from CpcSI. Located on preb3 plasmid. | 208 | 08-20-2012 |
| CpcS family | CMR092C | Cyanidioschyzon merolae | 10D | PC | 1 | long N-term compared to cyanobacterial CpcS sequences, found by tblastn | 405 | 02-21-2012 | |
| CpcS-I subfamily | gvip161 | NP_924137.1 | Gloeobacter violaceus | PCC7421 | PC+PEI | 2 | strange position in phylogenetic trees | 201 | 01-18-2018 |
| CpcS-I subfamily | Npun_F3809 | YP_001867136.1 | Nostoc punctiforme | ATCC29133 (PCC73102) | PC+PEI | 2 | CpcSIII in Schluchter et al., 2010? | 206 | 09-26-2011 |
| CpcS-I subfamily | ACCM5_010100032969 | ZP_09252138.1 | Acaryochloris sp. | CCMEE5410 | APC only | 196 | 08-24-2012 | ||
| CpcS-III subfamily | Mic7113_0798 | YP_007120112.1 | Microcoleus sp. | PCC7113 | PC+PEI | 2 | Found both CpcS-I and CpcS-III in this strain? | 188 | 06-20-2013 |
Model strains, Marine strains
Last update: 20 Apr 2023 14:39:21
