CyanoLyase

CpcT family

Description

Characterized in Synechococcus sp. PCC 7002 and Nostoc sp. PCC7120 to attach a PCB at Cys-153 of beta-PC (Shen et al., 2006; Zhao et al., 2007). Nostoc sp. PCC 7120 CpcT also attaches PCB to Cys 155 of PecB (i.e. beta-PEC; Zhao et al., 2007). Crystal structures of CpcT from Nostoc sp. PCC7120 and its complex with phycocyanobilin, have been obtained at 1.95 and 2.50 Å resolution, respectively (Zhou et al. 2014). CpcT forms a dimer and adopts a calyx-shaped beta-barrel fold.

Fold

Phyre2 found the following protein fold for this family:


c4o4oB_

References

  • Shen et al., 2006. Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta subunit of phycocyanin in Synechococcus sp. PCC 7002 J Biol Chem, 281, 17768-17778.
  • Zhao et al., 2007b. Lyase activities of CpcS- and CpcT-like proteins from Nostoc PCC7120 and sequential reconstitution of binding sites of phycoerythrocyanin and phycocyanin beta-subunits J Biol Chem, 282, 34093-34103.
  • Zhou et al., 2014. Structure and mechanism of the phycobilin lyase CpcT J Biol Chem, 289, 26677-26689.

Gene Locus tag Genbank Species Strain PBP in rods Pigment type Remark Length Last change
CpcT family WH7805_06621 ZP_01123324.1 Synechococcus sp. WH7805 PC+PEI 2 213 09-26-2011
CpcT family RS9917_02858 ZP_01080754.1 Synechococcus sp. RS9917 PC 1 197 09-26-2011
CpcT family RS9916_40316 DS022299.1 Synechococcus sp. RS9916 PC+PEI+PEII 3d/CA4 RS9916 possesses both cpcT and RpcT 198 05-27-2014
CpcT family WH5701_05945 ZP_01083809.1 Synechococcus sp. WH5701 PC 1 201 09-26-2011
CpcT family SynRCC307_2033 YP_001228289.1 Synechococcus sp. RCC307 PC+PEI+PEII 3b No rpcT 201 09-26-2011
CpcT family SCB01_010100012985 ZP_07974577.1 Synechococcus sp. CB0101 PC 1 197 11-04-2011
CpcT family CB0205_0884 Synechococcus sp. CB0205 PC+PEI 2 Gene was not modelled. Sequence not in refseq. 196 09-19-2012
CpcT family S7335_2352 ZP_05035920.1 Synechococcus sp. PCC7335 PC+PEI CA3 196 09-26-2011
CpcT family SYNPCC7002_A2095 YP_001735334.1 Synechococcus sp. PCC7002 PC 1 199 09-26-2011
CpcT family CPCC7001_2615 ZP_05046425.1 Cyanobium sp. PCC7001 PC 1 204 09-26-2011
CpcT family slr1649 NP_441787.1 Synechocystis sp. PCC6803 (ATCC27184) PC 1 196 09-26-2011
CpcT family AM1_C0119 YP_001521565.1 Acaryochloris marina MBIC11017 (AM1) PC 1 on preb3 plasmid 197 09-26-2011
CpcT family all5339 NP_489379.1 Anabaena (Nostoc) sp. PCC7120 PC+PEC Crystal structures of CpcT from Nostoc sp. PCC7120 and its complex with PCB were determined at 1.95 and 2.50 Å resolution, respectively (Zhou et al. 2014). 199 10-23-2014
CpcT family Ava_2579 YP_323089.1 Anabaena variabilis ATCC29413 (PCC7937) PC+PEC 199 09-27-2011
CpcT family Aazo_2275 YP_003721374.1 Anabaena (Nostoc) azollae 0708 PC+PEC 198 09-26-2011
CpcT family AmaxDRAFT_0741 ZP_03271923.1 Arthrospira (Spirulina) maxima CS-328 PC 1 198 09-26-2011
CpcT family CwatDRAFT_4238 ZP_00515713.1 Crocosphaera watsonii WH8501 PC+PEI 196 09-26-2011
CpcT family cce_1380 YP_001802796.1 Cyanothece sp. ATCC51142 PC 1 196 09-26-2011
CpcT family PCC7424_3679 YP_002378930.1 Cyanothece sp. PCC7424 PC+PEI 2 195 09-26-2011
CpcT family Cyan7425_0272 YP_002481027.1 Cyanothece sp. PCC7425 PC 1 196 09-26-2011
CpcT family PCC8801_1383 YP_002371599.1 Cyanothece sp. PCC8801 PC+PEI 2 196 09-26-2011
CpcT family Cyan8802_1417 YP_003137169.1 Cyanothece sp. PCC8802 PC 1 196 09-26-2011
CpcT family CY0110_13236 ZP_01728983.1 Cyanothece sp. CCY0110 PC 1 196 09-26-2011
CpcT family Cyan7822_5091 YP_003890251.1 Cyanothece sp. PCC7822 PC+PEI 2 195 09-26-2011
CpcT family FJSC11DRAFT_1806 ZP_08985600.1 Fischerella sp. (Mastigocladus laminosus) JSC-11 PC+PEC 196 11-04-2011
CpcT family glr1182 NP_924128.1 Gloeobacter violaceus PCC7421 PC+PEI 2 202 11-01-2012
CpcT family GL6909_5526 Gloeothece sp. PCC6909/1 PC+PEI 2 199 06-01-2011
CpcT family L8106_16979 ZP_01620427.1 Lyngbya aestuarii CCY9616 (PCC8106/CCY8106) PC 1 196 09-26-2011
CpcT family MC7420_6399 ZP_05026218.1 Microcoleus chthonoplastes PCC7420 PC+PEC 196 09-26-2011
CpcT family MAE_07320 YP_001655746.1 Microcystis aeruginosa NIES-843 PC 1 194 09-26-2011
CpcT family N9414_11904 ZP_01632483.1 Nodularia spumigena CCY9414 PC 1 196 09-26-2011
CpcT family Npun_F0744 YP_001864439.1 Nostoc punctiforme ATCC29133 (PCC73102) PC+PEI 2 197 09-26-2011
CpcT family Synpcc7942_0800 YP_399819.1 Synechococcus elongatus PCC7942 PC 1 197 08-28-2012
CpcT family syc0738_d YP_171448.1 Synechococcus elongatus PCC6301 (ATCC 27144) PC 1 197 09-26-2011
CpcT family CYB_2741 YP_478929.1 Synechococcus sp. JA-2-3B'a(2-13) PC 1 paralog of CYB_1028 199 09-17-2012
CpcT family CYA_2044 YP_475447.1 Synechococcus sp. JA-3-3Ab PC 1 199 08-24-2012
CpcT family tlr2156 NP_682946.1 Thermosynechococcus elongatus BP-1 PC 1 196 09-26-2011
CpcT family Tery_5043 YP_724425.1 Trichodesmium erythraeum IMS101 PC+PEI 195 09-26-2011
CpcT family CYA_2568 YP_475950.1 Synechococcus sp. JA-3-3Ab PC 1 Paralog of CYA_2044 198 08-24-2012
CpcT family CYB_1028 YP_477269.1 Synechococcus sp. JA-2-3B'a(2-13) PC 1 CpcT paralog 218 09-17-2012
CpcT family CRD_00828 ZP_06303865.1 Raphidiopsis brookii D9 PC 1 197 10-19-2011
CpcT family PCC_0629 YP_002049265.1 Paulinella chromatophora M0880 PC 1 201 09-19-2012
CpcT family CRC_00081 ZP_06306777.1 Cylindrospermopsis raciborskii CS-505 PC 1 197 10-19-2011
CpcT family OSCI_3240023 ZP_07111793.1 Oscillatoria sp. PCC6506 (PCC9029/ATCC29081 /UTEX 1547) PC 1 196 10-19-2011
CpcT family LYNGBM3L_15770 ZP_08426266.1 Lyngbya majuscula 3L PC+PEI 2 204 10-19-2011
CpcT family MicvaDRAFT_3520 ZP_08493427.1 Microcoleus vaginatus FGP-2 PC 1 196 10-20-2011
CpcT family IPF_3622 CAO90908.1 Microcystis aeruginosa PCC7806 PC 1 194 10-20-2011
CpcT family NIES39_A06470 BAI88485.1 Arthrospira (Spirulina) platensis NIES-39 PC 1 198 10-20-2011
CpcT family GTHECHR1104 XP_001713602.1 Guillardia theta CCMP2712 Cr-PE 545 n.a. Low protoscan score but best BLASTP match to the CpcT family. This cpcT-like gene was characterized by complementation of Synechocystis sp. PCC6803 cpcT (Bolte et al. 2008). However, since G. theta possesses PE (Cr-PE 545) not PC, the function of this gene (despite its closest similarity to cpcT than cpeT families) must likely be to attach a PEB at Cys-159 of beta-PE. 222 09-28-2012
CpcT family CMK263C Cyanidioschyzon merolae 10D PC 1 found by tblastn 263 02-21-2012
CpcT family Cy51472DRAFT_1191 ZP_08972395.1 Cyanothece sp. ATCC51472 PC 1 196 01-10-2012
CpcT family AplaP_010100001895 ZP_06380416.1 Arthrospira (Spirulina) platensis Paraca PC 1 198 01-24-2012
CpcT family HAN_1g134 XP_001712298.1 Hemiselmis andersenii CCMP644 Cr-PE 555 n.a. 221 01-24-2012
CpcT family CWATWH0003_2153 EHJ13154.1 Crocosphaera watsonii WH0003 PC+PEI 196 02-22-2012
CpcT family MICAI_2810005 ZP_10228696.1 Microcystis sp. T1-4 PC+PEI 2 194 08-22-2012
CpcT family MICAG_360013 CCI27632.1 Microcystis aeruginosa PCC9808 PC 1 194 08-22-2012
CpcT family MICAB_7270009 CCI00103.1 Microcystis aeruginosa PCC9717 PC 1 194 08-23-2012
CpcT family MICAK_250007 CCI36480.1 Microcystis aeruginosa PCC9701 PC 1 194 08-23-2012
CpcT family MICAC_2140007 CCI01493.1 Microcystis aeruginosa PCC9443 PC 1 194 08-23-2012
CpcT family MICAD_2070012 CCI06782.1 Microcystis aeruginosa PCC7941 PC 1 194 08-23-2012
CpcT family Nos7524_5510 YP_007078819.1 Nostoc sp. PCC7524 PC+PEC 198 04-23-2013
CpcT family Glo7428_1581 YP_007127302.1 Gloeocapsa sp. PCC7428 PC N-term shortened (21 aa) 196 07-02-2013
CpcT family Cylst_2544 YP_007147432.1 Cylindrospermum stagnale PCC7417 PC+PEC 198 04-23-2013
CpcT family Anacy_3145 YP_007157465.1 Anabaena cylindrica PCC7122 PC+PEC N-term shortened (12 aa) 200 07-02-2013
CpcT family Riv7116_4869 YP_007057827.1 Rivularia sp. PCC7116 PC+PEC 196 04-23-2013
CpcT family Chro_1061 YP_007090462.1 Chroococcidiopsis thermalis PCC7203 PC+PEC 197 04-23-2013
CpcT family Dacsa_1263 YP_007171317.1 Dactylococcopsis salina PCC8305 197 04-23-2013
CpcT family PCC7418_2907 YP_007169251.1 Halothece sp. PCC7418 PC 198 04-23-2013
CpcT family ZP_20934508 ZP_20934508.1 Microcystis aeruginosa TAIHU98 PC 1 194 04-23-2013
CpcT family ZP_18907915 ZP_18907915.1 Leptolyngbya sp. PCC7375 PC+PEI 2 196 04-23-2013
CpcT family Syn7509DRAFT_00027490 ZP_21040869.1 Synechocystis sp. PCC7509 PC 197 04-23-2013
CpcT family Osc7112_1339 YP_007114287.1 Oscillatoria nigro-viridis PCC7112 PC+PEI 2 196 04-23-2013
CpcT family Ple7327_1905 YP_007080807.1 Pleurocapsa sp. PCC7327 PC+PEI 196 04-23-2013
CpcT family Mic7113_5097 YP_007124160.1 Microcoleus sp. PCC7113 PC+PEI 2 196 04-23-2013
CpcT family Cyast_1789 YP_007165394.1 Cyanobacterium stanieri PCC7202 PC 194 04-23-2013
CpcT family GLO73106DRAFT_00031830 ZP_21049676.1 Gloeocapsa sp. PCC73106 PC+PEI 195 04-23-2013
CpcT family Xen7305DRAFT_00011110 ZP_21057060.1 Xenococcus sp. PCC7305 PC+PEI 194 04-23-2013
CpcT family Nos7107_3253 YP_007050989.1 Nostoc sp. PCC7107 PC+PEC 196 04-29-2013
CpcT family Cal7507_4092 YP_007067308.1 Calothrix sp. PCC7507 PC+PEC 199 04-29-2013
CpcT family Syn6312_0929 YP_007060674.1 Synechococcus sp. PCC6312 PC N-term shortened (14 aa) 198 07-02-2013
CpcT family GEI7407_0661 YP_007108211.1 Geitlerinema sp. PCC7407 PC 197 04-29-2013
CpcT family Cri9333_1163 YP_007141578.1 Crinalium epipsammum PCC9333 PC 196 04-29-2013
CpcT family Lep6406DRAFT_00020660 ZP_21046659.1 Leptolyngbya sp. PCC6406 PC+PEC 197 04-29-2013
CpcT family MICCA_210007 ZP_18814405.1 Microcystis aeruginosa PCC9432 PC 1 194 04-29-2013
CpcT family Cal6303_1725 YP_007136735.1 Calothrix sp. PCC6303 PC+PEI 2 197 04-29-2013
CpcT family Cha6605_4079 YP_007098562.1 Chamaesiphon minutus PCC6605 PC+PEI 203 04-29-2013
CpcT family Sta7437_3561 YP_007134025.1 Stanieria cyanosphaera PCC7437 PC+PEI 197 04-29-2013
CpcT family Cyan10605_2163 YP_007162293.1 Cyanobacterium aponimum PCC10605 194 04-29-2013
CpcT family Oscil6304_5951 YP_007089334.1 Oscillatoria acuminata PCC6304 (SAG 1449-3) PC 198 04-29-2013
CpcT family Cyagr_1634 YP_007046126.1 Cyanobium gracile PCC6307 PC 197 04-29-2013
CpcT family LepboDRAFT_3110 Leptolyngbya boryana PCC6306 PC 196 06-11-2013
CpcT family Fis9431DRAFT_1538 Fischerella sp. PCC9431 PC+PEC 196 06-12-2013
CpcT family FIS9605DRAFT_01268 Fischerella sp. PCC9605 PC+PEC 196 06-12-2013
CpcT family FisPCC73103_5898 Fischerella muscicola PCC73103 (SAG 1427-1) PC+PEC 196 06-12-2013
CpcT family Scytonema_PCC7110_joined_8455 Scytonema hofmanni PCC7110 PC+PEC 196 06-12-2013
CpcT family Osc10802DRAFT_6034 Oscillatoria sp. PCC10802 PC+PEI N-term shortened (22 aa) 198 07-02-2013
CpcT family PCC9339DRAFT_02937 Fischerella sp. PCC9339 PC+PEC 196 06-12-2013
CpcT family Ana7108_4567 Anabaena sp. PCC7108 PC+PEC 200 06-12-2013
CpcT family Chloroglopsis_PCC9212_joined_4222 Chlorogloeopsis sp. PCC9212 PC+PEC 195 06-12-2013
CpcT family UYC_07788 Chlorogloeopsis fritschii PCC6912 PC+PEC 196 06-12-2013
CpcT family FisPCC7414_5831 Fischerella muscicola PCC7414 PC+PEC 196 06-12-2013
CpcT family Fischerella_sp._PCC7521_5043 Fischerella thermalis PCC7521 PC+PEC 196 06-12-2013
CpcT family Pse7367_2699 YP_007103382.1 Pseudanabaena sp. PCC7367 PC+PEI Low protomatch score 202 06-20-2013
CpcT family Lepto7376_0226 YP_007069504.1 Leptolyngbya sp. PCC7376 PC+PEI 197 06-20-2013
CpcT family Pse7429DRAFT_1715 ZP_21065875.1 Pseudanabaena sp. (biceps) PCC7429 PC 214 06-20-2013
CpcT family Syn7502_02517 YP_007106625.1 Synechococcus sp. PCC7502 PC 197 06-20-2013

Model strain Model strains, Marine strains

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