E/F subclan 1 - Phycobilin lyases-isomerases
Description
This group gathers PecE/F, a PCB lyase isomerase found only in PEC-containing strains (Zhao et al., 2000, 2002, 2005; Storf et al., 2001) and RpcG, a fusion PEB lyase-isomerase found only in some PEII-containing marine strains (Synechococcus and Crocosphaera; Blot et al. 2009). In vitro, both PecE/F and RpcG can bind PCB and PEB on alpha-84 PC or PEI (Blot et al., 2009).
References
- Blot et al., 2009. Phycourobilin in Trichromatic Phycocyanin from Oceanic Cyanobacteria Is Formed Post-translationally by a Phycoerythrobilin Lyase-Isomerase J Biol Chem, 284, 9290-9298.
- Storf et al., 2001. Chromophore attachment to biliproteins: specificity of PecE/PecF, a lyase-isomerase for the photoactive 3(1)-cys-alpha 84-phycoviolobilin chromophore of phycoerythrocyanin Biochemistry, 40, 12444-12456.
- Zhao et al., 2000. Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon FEBS Lett, 469, sept-13.
- Zhao et al., 2002. Characterization of phycoviolobilin phycoerythrocyanin-alpha 84-cystein-lyase-(isomerizing) from Mastigocladus laminosus Eur J Biochem, 269, 4542-4550.
- Zhao et al., 2005. Amino acid residues associated with enzymatic activities of the isomerizing phycoviolobilin-lyase PecE/F Biochemistry, 44, 8126-8137.
Showing only unsure genes
| Gene | Locus tag | Genbank | Species | Strain | PBP in rods | Pigment type | Remark | Length | Last change |
|---|---|---|---|---|---|---|---|---|---|
| PecF family | Aazo_0293 | YP_003720001.1 | Anabaena (Nostoc) azollae | 0708 | PC+PEC | Sequence with an N-term shorter than others (seemingly lacks about 22 otherwise conserved aa) but no obvious problem in genome sequence. The missing N-term contains 3 or 4 His in other strains. | 151 | 08-28-2012 |
Model strains, Marine strains
Last update: 19 Jan 2018 15:00:53
